From Brotato Wiki
Game Challenges
Danger 0 - Win a run in Danger 0 - Unlocks 
Danger 1 - Win a run in Danger 1 - Unlocks 
Danger 2 - Win a run in Danger 2 - Unlocks 
Danger 3 - Win a run in Danger 3 - Unlocks 
Danger 4 - Win a run in Danger 4 - Unlocks 
Danger 5 - Win a run in Danger 5 - Unlocks 
Survivor 1 - Kill 300 enemies - Unlocks 
Survivor 2 - Kill 2000 enemies - Unlocks 
Survivor 3 - Kill 5000 enemies - Unlocks 
Survivor 4 - Kill 10000 enemies - Unlocks 
Survivor 5 - Kill 20000 enemies - Unlocks 
Gatherer 1 - Collect 300 materials - Unlocks 
Gatherer 2 - Collect 2000 materials - Unlocks 
Gatherer 3 - Collect 5000 materials - Unlocks 
Gatherer 4 - Collect 10000 materials - Unlocks 
Gatherer 5 - Collect 20000 materials - Unlocks 
Rookie - Die for the first time - Unlocks 
Dying - Reach -5% HP Regeneration - Unlocks 
Agriculture - Reach +200 Harvesting - Unlocks 
Hallucination - Reach +60% Dodge - Unlocks 
Hallucination - Reach +50% Speed - Unlocks 
Hoarder - Hold 3000 Materials - Unlocks 
Builder - Get 5 turrets on the map simultaneously - Unlocks 
Lumberjack - Kill 50 trees - Unlocks 
Medicine - Heal 200 HP in one wave- Unlocks 
Hero Challenges
Brawler - Win a run with Brawler - Unlocks 
Bull - Win a run with Bull - Unlocks 
Chunky - Win a run with Chunky - Unlocks 
Crazy - Win a run with Crazy - Unlocks 
Demon - Win a run with Demon - Unlocks 
Doctor - Win a run with Doctor - Unlocks 
Engineer - Win a run with Engineer - Unlocks 
Entrepreneur - Win a run with Entrepreneur - Unlocks 
Explorer - Win a run with Explorer - Unlocks 
Farmer - Win a run with Farmer - Unlocks 
Generalist - Win a run with Generalist - Unlocks 
Ghost - Win a run with Ghost - Unlocks 
Gladiator - Win a run with Gladiator - Unlocks 
Knight - Win a run with Knight - Unlocks 
Loud - Win a run with Loud - Unlocks 
Lucky - Win a run with Lucky - Unlocks 
Mage - Win a run with Mage - Unlocks 
Masochist - Win a run with Masochist - Unlocks 
Multitasker - Win a run with Multitasker - Unlocks 
Mutant - Win a run with Mutant - Unlocks 
Old - Win a run with Old - Unlocks 
One Armed - Win a run with One Armed - Unlocks 
Pacifist - Win a run with Pacifist - Unlocks 
Ranger - Win a run with Ranger - Unlocks 
Saver - Win a run with Saver - Unlocks 
Sick - Win a run with Sick - Unlocks 
Soldier - Win a run with Soldier - Unlocks 
Speedy - Win a run with Speedy - Unlocks 
Well Rounded - Win a run with Well Rounded - Unlocks 
Wildling - Win a run with Wildling - Unlocks 